Manioc : Portail | resultats 1 - 20 pour la recherche: Davis, Angela

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

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Une histoire des colonies anglaises aux Antilles: de 1649 à 1660 : thèse présentée à la faculté des lettres de l'Université de Paris
Auteur(s) : Watts, Arthur Pryor (18..-19..)
Auteurs secondaires : Université de Paris (1896-1968). Faculté des lettres
Année de publication :

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The conserved histone deacetylase Rpd3 and its DNA binding subunit Ume6 control dynamic transcript architecture during mitotic growth and meiotic development.
Auteur(s) : Lardenois, Aurélie Stuparevic, Igor Liu, Yuchen Law, Michael J Becker, Emmanuelle Smagulova, Fatima Waern, Karl Guilleux, Marie-Hélène
Auteurs secondaires : Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) Rowan University Stanford University [Stanford] Stanford Genome Technology Center ; Stanford Genome Technology Center European Molecular Biology Laboratory [Heidelberg] (EMBL) Fondation pour la Recherche Medicale 4th year PhD fellowship [FDT20100920148 to Y.L.]; Institut National de Santé et de Recherche Medicale (Inserm) Avenir [R07216NS]; La Région de Bretagne CREATE [NR11016NN]; SAD [R13124NN to M.P.]; National Institutes of Health [GMO68717 to L.M.S., 5P01HG000205 to R.W.D., GM082013 to M.L.]; Deutsche Forschungs Gemeinschaft [1422/3-1 to L.M.S.]. Funding for open access charge: Institut National de Santé et de Recherche Medicale (Inserm).

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Excursion dans l'Eldorado : El Callao
Auteur(s) : Morisse, Lucien
Année de publication :

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